"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.
Identifieur interne : 000286 ( Main/Exploration ); précédent : 000285; suivant : 000287"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.
Auteurs : Akihiko Nakamura [Japon] ; Takuya Ishida [Japon] ; Katsuhiro Kusaka [Japon] ; Taro Yamada [Japon] ; Shinya Fushinobu [Japon] ; Ichiro Tanaka [Japon] ; Satoshi Kaneko [Japon] ; Kazunori Ohta [Japon] ; Hiroaki Tanaka [Japon] ; Koji Inaka [Japon] ; Yoshiki Higuchi [Japon] ; Nobuo Niimura [Japon] ; Masahiro Samejima [Japon] ; Kiyohiko Igarashi [Japon]Source :
- Science advances [ 2375-2548 ] ; 2015.
Abstract
Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray diffraction analyses to investigate the hydrogen bond network in inverting cellulase PcCel45A, which is an endoglucanase belonging to subfamily C of GH family 45, isolated from the basidiomycete Phanerochaete chrysosporium. Examination of the enzyme and enzyme-ligand structures indicates a key role of multiple tautomerizations of asparagine residues and peptide bonds, which are finally connected to the other catalytic residue via typical side-chain hydrogen bonds, in forming the "Newton's cradle"-like proton relay pathway of the catalytic cycle. Amide-imidic acid tautomerization of asparagine has not been taken into account in recent molecular dynamics simulations of not only cellulases but also general enzyme catalysis, and it may be necessary to reconsider our interpretation of many enzymatic reactions.
DOI: 10.1126/sciadv.1500263
PubMed: 26601228
PubMed Central: PMC4643802
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.</title><author><name sortKey="Nakamura, Akihiko" sort="Nakamura, Akihiko" uniqKey="Nakamura A" first="Akihiko" last="Nakamura">Akihiko Nakamura</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ishida, Takuya" sort="Ishida, Takuya" uniqKey="Ishida T" first="Takuya" last="Ishida">Takuya Ishida</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kusaka, Katsuhiro" sort="Kusaka, Katsuhiro" uniqKey="Kusaka K" first="Katsuhiro" last="Kusaka">Katsuhiro Kusaka</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106</wicri:regionArea><wicri:noRegion>Ibaraki 319-1106</wicri:noRegion></affiliation></author><author><name sortKey="Yamada, Taro" sort="Yamada, Taro" uniqKey="Yamada T" first="Taro" last="Yamada">Taro Yamada</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106</wicri:regionArea><wicri:noRegion>Ibaraki 319-1106</wicri:noRegion></affiliation></author><author><name sortKey="Fushinobu, Shinya" sort="Fushinobu, Shinya" uniqKey="Fushinobu S" first="Shinya" last="Fushinobu">Shinya Fushinobu</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tanaka, Ichiro" sort="Tanaka, Ichiro" uniqKey="Tanaka I" first="Ichiro" last="Tanaka">Ichiro Tanaka</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Research Center for 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K" first="Kazunori" last="Ohta">Kazunori Ohta</name><affiliation wicri:level="1"><nlm:affiliation>Japanese Experiment Module (JEM) Utilization Center, Japan Aerospace Exploration Agency, Ibaraki 305-8505, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Japanese Experiment Module (JEM) Utilization Center, Japan Aerospace Exploration Agency, Ibaraki 305-8505</wicri:regionArea><wicri:noRegion>Ibaraki 305-8505</wicri:noRegion></affiliation></author><author><name sortKey="Tanaka, Hiroaki" sort="Tanaka, Hiroaki" uniqKey="Tanaka H" first="Hiroaki" last="Tanaka">Hiroaki Tanaka</name><affiliation wicri:level="1"><nlm:affiliation>Confocal Science Inc., Tokyo 101-0032, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Confocal Science Inc., Tokyo 101-0032</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Inaka, Koji" sort="Inaka, Koji" uniqKey="Inaka K" first="Koji" last="Inaka">Koji Inaka</name><affiliation wicri:level="1"><nlm:affiliation>Maruwa Foods and Biosciences Inc., Nara 639-1123, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Maruwa Foods and Biosciences Inc., Nara 639-1123</wicri:regionArea><wicri:noRegion>Nara 639-1123</wicri:noRegion></affiliation></author><author><name sortKey="Higuchi, Yoshiki" sort="Higuchi, Yoshiki" uniqKey="Higuchi Y" first="Yoshiki" last="Higuchi">Yoshiki Higuchi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Life Science, University of Hyogo, Hyogo 678-1297, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Life Science, University of Hyogo, Hyogo 678-1297</wicri:regionArea><wicri:noRegion>Hyogo 678-1297</wicri:noRegion></affiliation></author><author><name sortKey="Niimura, Nobuo" sort="Niimura, Nobuo" uniqKey="Niimura N" first="Nobuo" last="Niimura">Nobuo 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sort="Nakamura, Akihiko" uniqKey="Nakamura A" first="Akihiko" last="Nakamura">Akihiko Nakamura</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ishida, Takuya" sort="Ishida, Takuya" uniqKey="Ishida T" first="Takuya" last="Ishida">Takuya Ishida</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kusaka, Katsuhiro" sort="Kusaka, Katsuhiro" uniqKey="Kusaka K" first="Katsuhiro" last="Kusaka">Katsuhiro Kusaka</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</nlm:affiliation><country 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and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tanaka, Ichiro" sort="Tanaka, Ichiro" uniqKey="Tanaka I" first="Ichiro" last="Tanaka">Ichiro Tanaka</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106</wicri:regionArea><wicri:noRegion>Ibaraki 319-1106</wicri:noRegion></affiliation></author><author><name sortKey="Kaneko, Satoshi" sort="Kaneko, Satoshi" uniqKey="Kaneko S" first="Satoshi" last="Kaneko">Satoshi Kaneko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Subtropical Biochemistry and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Subtropical Biochemistry and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213</wicri:regionArea><wicri:noRegion>Okinawa 903-0213</wicri:noRegion></affiliation></author><author><name sortKey="Ohta, Kazunori" sort="Ohta, Kazunori" uniqKey="Ohta K" first="Kazunori" last="Ohta">Kazunori Ohta</name><affiliation wicri:level="1"><nlm:affiliation>Japanese Experiment Module (JEM) Utilization Center, Japan Aerospace Exploration Agency, Ibaraki 305-8505, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Japanese Experiment Module (JEM) Utilization Center, Japan Aerospace Exploration Agency, Ibaraki 305-8505</wicri:regionArea><wicri:noRegion>Ibaraki 305-8505</wicri:noRegion></affiliation></author><author><name sortKey="Tanaka, Hiroaki" sort="Tanaka, Hiroaki" uniqKey="Tanaka H" first="Hiroaki" last="Tanaka">Hiroaki Tanaka</name><affiliation wicri:level="1"><nlm:affiliation>Confocal Science Inc., Tokyo 101-0032, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Confocal Science Inc., Tokyo 101-0032</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Inaka, Koji" sort="Inaka, Koji" uniqKey="Inaka K" first="Koji" last="Inaka">Koji Inaka</name><affiliation wicri:level="1"><nlm:affiliation>Maruwa Foods and Biosciences Inc., Nara 639-1123, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Maruwa Foods and Biosciences Inc., Nara 639-1123</wicri:regionArea><wicri:noRegion>Nara 639-1123</wicri:noRegion></affiliation></author><author><name sortKey="Higuchi, Yoshiki" sort="Higuchi, Yoshiki" uniqKey="Higuchi Y" first="Yoshiki" last="Higuchi">Yoshiki Higuchi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Life Science, University of Hyogo, Hyogo 678-1297, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Life Science, University of Hyogo, Hyogo 678-1297</wicri:regionArea><wicri:noRegion>Hyogo 678-1297</wicri:noRegion></affiliation></author><author><name sortKey="Niimura, Nobuo" sort="Niimura, Nobuo" uniqKey="Niimura N" first="Nobuo" last="Niimura">Nobuo Niimura</name><affiliation wicri:level="1"><nlm:affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106</wicri:regionArea><wicri:noRegion>Ibaraki 319-1106</wicri:noRegion></affiliation></author><author><name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j">Science advances</title><idno type="ISSN">2375-2548</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray diffraction analyses to investigate the hydrogen bond network in inverting cellulase PcCel45A, which is an endoglucanase belonging to subfamily C of GH family 45, isolated from the basidiomycete Phanerochaete chrysosporium. Examination of the enzyme and enzyme-ligand structures indicates a key role of multiple tautomerizations of asparagine residues and peptide bonds, which are finally connected to the other catalytic residue via typical side-chain hydrogen bonds, in forming the "Newton's cradle"-like proton relay pathway of the catalytic cycle. Amide-imidic acid tautomerization of asparagine has not been taken into account in recent molecular dynamics simulations of not only cellulases but also general enzyme catalysis, and it may be necessary to reconsider our interpretation of many enzymatic reactions. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">26601228</PMID><DateCompleted><Year>2015</Year><Month>11</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2375-2548</ISSN><JournalIssue CitedMedium="Print"><Volume>1</Volume><Issue>7</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Science advances</Title><ISOAbbreviation>Sci Adv</ISOAbbreviation></Journal><ArticleTitle>"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.</ArticleTitle><Pagination><MedlinePgn>e1500263</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1126/sciadv.1500263</ELocationID><Abstract><AbstractText>Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray diffraction analyses to investigate the hydrogen bond network in inverting cellulase PcCel45A, which is an endoglucanase belonging to subfamily C of GH family 45, isolated from the basidiomycete Phanerochaete chrysosporium. Examination of the enzyme and enzyme-ligand structures indicates a key role of multiple tautomerizations of asparagine residues and peptide bonds, which are finally connected to the other catalytic residue via typical side-chain hydrogen bonds, in forming the "Newton's cradle"-like proton relay pathway of the catalytic cycle. Amide-imidic acid tautomerization of asparagine has not been taken into account in recent molecular dynamics simulations of not only cellulases but also general enzyme catalysis, and it may be necessary to reconsider our interpretation of many enzymatic reactions. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nakamura</LastName><ForeName>Akihiko</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ishida</LastName><ForeName>Takuya</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kusaka</LastName><ForeName>Katsuhiro</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yamada</LastName><ForeName>Taro</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fushinobu</LastName><ForeName>Shinya</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tanaka</LastName><ForeName>Ichiro</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaneko</LastName><ForeName>Satoshi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Subtropical Biochemistry and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ohta</LastName><ForeName>Kazunori</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Japanese Experiment Module (JEM) Utilization Center, Japan Aerospace Exploration Agency, Ibaraki 305-8505, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tanaka</LastName><ForeName>Hiroaki</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Confocal Science Inc., Tokyo 101-0032, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Inaka</LastName><ForeName>Koji</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Maruwa Foods and Biosciences Inc., Nara 639-1123, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Higuchi</LastName><ForeName>Yoshiki</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Life Science, University of Hyogo, Hyogo 678-1297, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niimura</LastName><ForeName>Nobuo</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Frontier Research Center for Applied Atomic Sciences, Ibaraki University, Ibaraki 319-1106, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Samejima</LastName><ForeName>Masahiro</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Igarashi</LastName><ForeName>Kiyohiko</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Sci Adv</MedlineTA><NlmUniqueID>101653440</NlmUniqueID><ISSNLinking>2375-2548</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Carbohydrate Active enZymes</Keyword><Keyword MajorTopicYN="N">Cellulase</Keyword><Keyword MajorTopicYN="N">Neutron crystallography</Keyword><Keyword MajorTopicYN="N">Phanerochaete chrysosporium</Keyword><Keyword MajorTopicYN="N">Proton relay</Keyword><Keyword MajorTopicYN="N">Tautomerization</Keyword><Keyword MajorTopicYN="N">biomass</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>03</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>06</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26601228</ArticleId><ArticleId 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last="Nakamura">Akihiko Nakamura</name></region><name sortKey="Fushinobu, Shinya" sort="Fushinobu, Shinya" uniqKey="Fushinobu S" first="Shinya" last="Fushinobu">Shinya Fushinobu</name><name sortKey="Higuchi, Yoshiki" sort="Higuchi, Yoshiki" uniqKey="Higuchi Y" first="Yoshiki" last="Higuchi">Yoshiki Higuchi</name><name sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name><name sortKey="Inaka, Koji" sort="Inaka, Koji" uniqKey="Inaka K" first="Koji" last="Inaka">Koji Inaka</name><name sortKey="Ishida, Takuya" sort="Ishida, Takuya" uniqKey="Ishida T" first="Takuya" last="Ishida">Takuya Ishida</name><name sortKey="Kaneko, Satoshi" sort="Kaneko, Satoshi" uniqKey="Kaneko S" first="Satoshi" last="Kaneko">Satoshi Kaneko</name><name sortKey="Kusaka, Katsuhiro" sort="Kusaka, Katsuhiro" uniqKey="Kusaka K" first="Katsuhiro" last="Kusaka">Katsuhiro Kusaka</name><name sortKey="Niimura, Nobuo" sort="Niimura, Nobuo" uniqKey="Niimura N" first="Nobuo" last="Niimura">Nobuo Niimura</name><name sortKey="Ohta, Kazunori" sort="Ohta, Kazunori" uniqKey="Ohta K" first="Kazunori" last="Ohta">Kazunori Ohta</name><name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name><name sortKey="Tanaka, Hiroaki" sort="Tanaka, Hiroaki" uniqKey="Tanaka H" first="Hiroaki" last="Tanaka">Hiroaki Tanaka</name><name sortKey="Tanaka, Ichiro" sort="Tanaka, Ichiro" uniqKey="Tanaka I" first="Ichiro" last="Tanaka">Ichiro Tanaka</name><name sortKey="Yamada, Taro" sort="Yamada, Taro" uniqKey="Yamada T" first="Taro" last="Yamada">Taro Yamada</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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